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Título : | Muscarinic agents modify kinetics properties of membrane-bound guanylyl cyclase activity I. |
Autor : | Alfonzo R., Marcelo J. Gonzalez de Alfonzo, Ramona Lippo de Becemberg, Itala Pefia de Aguilar, A.E. Camarillo, I |
Palabras clave : | membrane-bound guanylyl cyclase Muscarinic agents NaCI inhibition Muscarinic receptor Enzyme-receptor complex Plasma membrane Tracheal smooth muscle |
Fecha de publicación : | Aug-1989 |
Editorial : | FEBS LETTERS |
Citación : | Vol. 253;1,2 |
Resumen : | Plasma membranes from bovine tracheal smooth muscle show guanylyl cyclase activity, which can be stimulated by muscarinic
agonists such carbamylcholine and oxotremorine and blocked by atropine. This stimulation was observed in the
presence of 150 mM NaCI. In the absence of this salt, guanylyl cyclase activity was considerably higher but was not
affected by muscarinic agonists. Carbamylcholine decreased the apparent K,~ but did not change the Vm~ of this enzyme.
When plasma membrane fractions were extracted with 1% octylglucoside, guanylyl cyclase activity was preserved, however
the muscarinic activation was abolished, despite a muscarinic receptor capable of [aH]quinuclidinylbenzilate binding
being present in the extract. The detergent extraction changed the affinity of guanylyl cyclase for GTP but the Mn z+
kinetics was unaltered. Based on these findings and on current information in the literature, we propose that another
component is required to restore the link between the muscarinic receptor and guanylyl cyclase, however the nature of
this component remains to be established. |
URI : | http://hdl.handle.net/10872/13239 |
ISSN : | 0014-5793 |
Aparece en las colecciones: | Artículos Publicados
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