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Título : Concordance between IFNγ +874 A/T polymorphism and interferon-γ expression in a TB-endemic indigenous settingConcordance between IFNγ +874 A/T polymorphism and interferon-γ expression in a TB-endemic indigenous setting
Autor : Araujo, Zaida
Palacios, Andrea
Biomon, Ruben
Rivas-Santiago, Bruno
Serrano, Carmen Judith
Enciso-Moreno, Leonor
Lopez-Ramos, Juan-Ernesto
Wide, Albina
Jiménez, Juan Carlos
Fernandez De Larrea, Carlos
Enciso-Moreno, Jose Antonio
Palabras clave : Warao
TB
IFNG +874 A/T polymorphism
Genotype
Allele
Fecha de publicación : Apr-2017
Editorial : Revista da Sociedade Brasileira de Medicina Tropica
Citación : Vol. 50;2
Resumen : Interferon-γ (IFN-γ) plays a crucial role in resistance to mycobacterial diseases; accordingly, variants of the gene encoding this cytokine may be associated with elevated risk of contracting pulmonary tuberculosis (TB). Methods: Blood samples were collected from 135 Warao indigenous individuals with newly diagnosed sputum culture-positive TB. Of these, 24 were diagnosed with active tuberculosis (ATB). The study comprised 111 participants, who were grouped as follows: 1) 14 tuberculin skin test (TST)-positive Warao indigenous individuals and 4 that were QuantiFERON-TBGold In-Tube (QFT-IT) test-positive, collectively comprising the latent TB infection group (LTBI), n = 18), and 2) healthy controls who were QFT-ITand TST-negative, comprising the control group (CTRL, n = 93). Detection of the IFNG+874A/T polymorphism was performed via PCR and quantification of IFN-γ gene (IFNG) expression via qPCR. Results: Relative to indigenous and white Americans, ATB and CTRL groups had a higher frequency of the IFNG SNP (+874A): 23 (95.8%) and 108 (97.3%), respectively. Indigenous Warao individuals homozygous for the IFNG (+874) A allele exhibited 3.59-fold increased risk of developing TB (95% confidence interval, 2.60-4.96, p =0.0001). A decreased frequency of the AT genotype was observed in individuals with pulmonary TB (4.16%) and controls (0.90%). The frequency of the TT genotype was decreased among controls (1.80%); none of the patients with TB were found to have this genotype. The differences in IFNG expression between the groups, under unstimulated and stimulated conditions, were not statistically significant.
URI : http://hdl.handle.net/10872/16471
ISSN : 0037-8682
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