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Título : | Evaluation of the transcriptional immune biomarkers in peripheral blood from Warao indigenous associate with the infection by Mycobacterium tuberculosis |
Autor : | Araujo, Zaida Palacios, Andrea Enciso-Moreno, Leonor Lopez-Ramos, Juan Ernesto Wide, Albina De Waard, Jacobus Rivas-Santiago, Bruno Serrano, Carmen Bastian-Hernandez, Yadira Enciso-Moreno, Jose |
Palabras clave : | Tuberculosis. Warao indigenous Immune biomarker Real-time quantitative PCR (qPCR). |
Fecha de publicación : | 2019 |
Editorial : | Revista da Sociedade Brasileira de Medicina Tropical |
Citación : | Araujo. Z Et All Evaluation of the transcriptional immune biomarkers in peripheral blood from Warao indigenous associate with the infection by Mycobacterium tuberculosis Vol. 52 2019 |
Citación : | Vol.;52 |
Resumen : | Introduction: Biomarkers are critical tools for finding new approaches for controlling the spread of tuberculosis (TB), including
for predicting the development of TB therapeutics, vaccines, and diagnostic tools. Methods: We analyzed transcriptional immune
biomarkers ex vivo and in M. tuberculosis antigens (ESAT-6, CFP10, and TB7.7) in stimulated peripheral blood of Warao indigenous individuals. These biomarkers may be able to differentiate TB states, such as active tuberculosis (ATB) cases and latent tuberculosis infection (LTBI) from non-infected controls (NIC). A real-time reverse transcription polymerase chain reaction (RT-qPCR) assay was
performed on 100 blood samples under non-stimulation or direct ex vivo conditions (NS=50) and stimulation conditions (S=50).
Results: The findings are shown as the median and interquartile range (IQR) of relative gene expression levels of IFN-γ, CD14, MMP9,
CCR5, CCL11, CXCL9/MIG, and uPAR/PLAUR immune biomarkers. MMP9 levels were significantly higher in the LTBI-NS and LTBI-S groups compared with the NIC-NS and NIC-S groups. However, CCR5 levels were significantly lower in the LTBI-S group compared with both NIC-NS and NIC-S groups. CCL11 levels were significantly lower in the LTBI-S group compared with the NIC- NS group. Conclusions: Preliminary findings showed that MMP9 immune biomarkers separated LTBI indigenous individuals from NIC indigenous individuals, while CCR5, CCL11, CD14, and IFN-γ did not differentiate TB states from NIC. MMP9 may be useful as a potential biomarker for LTBI and new infected case detection among Warao indigenous individuals at high risk of developing the disease. It may also be used to halt the epidemic, which will require further validation in larger studies. |
URI : | http://hdl.handle.net/10872/19841 |
ISSN : | 1678-9849 |
Aparece en las colecciones: | Artículos Publicados
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