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Título : | Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue |
Otros títulos : | Producto de Excreción de Shigella dysenteriae y la Muerte Celular por Apoptosis en Tejido Muscular de Embrión de Pollo |
Autor : | Alvarez, Marco Urbina, Gidalia Müller, Claudia Perdomo, Lourdes |
Palabras clave : | Shigella dysenteriae; Shiga toxin; Chick embryo; Muscle tissue; Apoptosis |
Fecha de publicación : | 12-Nov-2013 |
Citación : | Int.Journal.Morphology;25(3) |
Resumen : | SUMMARY: The aim of this study was to evaluate the acute cell injury of excretion products present in culture filtrate from
Shigella dysenteriae in both whole lower limb of chick embryo ex vivo and myoblasts cells developed in hanging-drop cultures in vitro.
Three controls were defined: a) Tyrode’s solution b) brain-heart broth infusion (CCC) and c) supernatant not toxigenic of E.coli
O157:H7. Shigella dysenteriae were culture for 24 hours and the excretion products were obtained after centrifugation of the culture.
After 1h of treatment, the morphologic changes in limbs treated with raw filtrate were evaluated through histopathological examination
of sections stained with hematoxylin-eosin (H&E-stain) and Gomori’s trichrome by image analysis techniques. Quantification of apoptotic
cells was measured by an enzyme-linked immunoassay TUNEL. The morphological feature of apoptosis were evaluated in culture
myoblasts. In contrast with controls, the longitudinal section on treated thigh of chick embryo limb-buds show atrophy muscle tissue,
detachment of few fibers, 57,14% decrease in the number of cells, and loss of collagen substrate. Apoptotic index percent increase and
mitotic index decrease in response to excretion products were observed, but were not significant. Membrane blebbing, vacuolation, small
aggregates of chromatin around the nucleus and loss of cell adhesion were observed. Culture filtrate from Shigella dysenteriae produced
cytotoxic effect on cell of muscle fibers with acute cell injuries suspected to be related to the apoptotic cell death. |
URI : | http://hdl.handle.net/10872/4801 |
Aparece en las colecciones: | Artículos Publicados
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